On the Use of Tritium-labelled Albumin for Studies of Intestinal Absorption.

The Absorption of Tritium-labelled Pyridoxine Hcl in Control Subjects and in Patients with Intestinal Malabsorption.

The assay of tritium-labelled folate in faeces by the oxygen flask combustion technique followed by liquid scintillation counting.

The assay of tritium-labelled substances in faeces requires conversion of tritium into either tritium gas (Payne and Done, 1958) or tritium-labelled water. Belcher (1961) and Kremenchuzky, Musso, Hoffbrand, and Viola (1967) used wet oxidation of faeces followed by liquid scintillation counting, while Burns and Glass (1963) used dry oxidation in a bomb calorimeter. There is a need for a reproduc...

Fractional clearance of albumin is influenced by its degradation during renal passage.

1. The fractional clearance of intact albumin as determined by fractionation of urine by gel chromatography gave a value of 3.9 +/- 1.6 x 10(-4) for the isolated perfused kidney and 2.1 +/- 0.6 x 10(-4) in vivo using ALZET osmotic pumps. 2. Albumin fractional clearance as measured by detection of the tritium label on the albumin molecule by radioactivity analysis gave a value of 7.5 +/- 3.9 x 1...

Cell penetrating and transytosing peptides: powerful strategies for oral insulin delivery

 Insulin is essential for type 1 and advanced type 2 diabetes to maintain blood glucose levels and increase the patient’s longevity. Frequent subcutaneous insulin injections are usually associated with pain, local tissue necrosis, infection and nerve damage. Recently, a number of new delivery methods such as oral insulin delivery have been developed to overcome these limitations and increase pa...

Biophysical and Molecular Docking Studies of Human Serum Albumin Interactions with a Potential Anticancer Pt(II) Complex

The interaction between [Pt(phen)(pyrr-dtc)]NO3 (where phen = 1,10-phenanthroline and pyrr-dtc =pyrrolidinedithiocarbamat) with human serum albumin (HSA) was studied by fluorescence, UV–vis absorption, circular dichroism (CD) spectroscopy and molecular docking technique under like physiological condition in Tris–HCl buffer solution at pH 7.4. UV-Vis absorption spectroscopy indicates that the pro...